Journal: Molecular Medicine Reports

Article Title: Long non-coding RNA ZFPM2-AS1 promotes colorectal cancer progression by sponging miR-137 to regulate TRIM24

doi: 10.3892/mmr.2020.11737

Figure Lengend Snippet: miR-137 is a direct target of ZFPM2-AS1. (A) Predicted binding site of miR-137 in ZFPM2-AS1. (B) miR-137 expression in SW480 and HCT116 cells transfected with sh-ZFPM2-AS1. (C) miR-137 expression in SW480 and HCT116 cells following transfection with miR-137 mimics or miR-137 inhibitor. (D) Effects of miR-137 mimics on luciferase activity WT or MUT ZFPM2-AS1 gene constructs. (E) Radioimmunoprecipitation and reverse transcription-quantitative PCR analysis of the binding between ZFPM2-AS1 and miR-137. (F) miR-137 expression in CRC tumor tissues and in adjacent normal tissues. (G) miR-137 expression in CRC cell lines. (H) Correlation between ZFPM2-AS1 and miR-137 expressions in CRC tissues. (I and J) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on proliferation of SW480 and HCT116 cells. (K) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on cell migration and invasion of SW480 and HCT116 cells (scale bar, 200 µm). **P<0.01, ***P<0.001 vs. control group. miR, microRNA; CRC, colorectal cancer; sh, short hairpin; NC, negative control; OD, optical density; WT, wild-type; MUT, mutant; Ig, immunoglobulin; Ago2, Argonaute 2.

Article Snippet: Reverse transcription protocol consisted of 37°C for 15 min, followed by 85°C for 5 sec. To determine miRNA expression levels, cDNA was synthesized using the qScript microRNA cDNA Synthesis kit (Guangzhou RiboBio Co., Ltd.).

Techniques: Binding Assay, Expressing, Transfection, Luciferase, Activity Assay, Construct, Real-time Polymerase Chain Reaction, Migration, Negative Control, Mutagenesis

Journal: Molecular Medicine Reports

Article Title: Long non-coding RNA ZFPM2-AS1 promotes colorectal cancer progression by sponging miR-137 to regulate TRIM24

doi: 10.3892/mmr.2020.11737

Figure Lengend Snippet: TRIM24 is a direct target of miR-137. (A) Predicted binding site of miR-137 in TRIM24. (B) TRIM24 mRNA expression in CRC tumor and adjacent normal tissue. (C) TRIM24 mRNA expression in CRC cell lines. (D) TRIM24 mRNA and (E) protein expression following transfection with miR-137 mimics or miR-137 inhibitor in SW480 and HCT116 cells. (F) Effects of miR-137 mimics on luciferase activity of WT or MUT TRIM24 constructs. (G) Correlation between TRIM24 and miR-137 expressions in CRC tissues. (H) TRIM24 mRNA and (I) protein expression following transfection with sh-ZFPM2-AS1 and miR-137 inhibitor in SW480 and HCT116 cells. **P<0.01, ***P<0.001 vs. control group. miR, microRNA; CRC, colorectal cancer; TRIM24, tripartite motif containing 24; sh, short hairpin; NC, negative control; WT, wild-type; MUT, mutant.

Article Snippet: Reverse transcription protocol consisted of 37°C for 15 min, followed by 85°C for 5 sec. To determine miRNA expression levels, cDNA was synthesized using the qScript microRNA cDNA Synthesis kit (Guangzhou RiboBio Co., Ltd.).

Techniques: Binding Assay, Expressing, Transfection, Luciferase, Activity Assay, Construct, Negative Control, Mutagenesis

Journal: PLoS ONE

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae

doi: 10.1371/journal.pone.0182726

Figure Lengend Snippet: Sequences of European eel ( Anguilla anguilla ) primers used for amplification of genes by qRT-PCR. Primers were designed from predicted or cloned cDNA sequences available in Genbank nucleotide and WGS databases. The table lists accession number and corresponding database of target gene sequences.

Article Snippet: From the resulting total RNA, 680 ng were transcribed using the Quanta qScript cDNA Synthesis Kit (Promega, Germany) according to the manufacturer’s instructions, including an additional gDNA wipe out step prior to transcription [Quanta PerfeCta DNase I Kit (Promega, Germany)].

Techniques: Amplification, Clone Assay, Sequencing